Results
| PMID | 17706207 |
| Gene Name | KLRD1 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 33 |
| Population details | 33 (20 with endometriosis, 13 without endometriosis) |
| Sex | Female |
| Associated genes | CD94/NKG2A, HLA-E |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2008 May;89(5 Suppl):1490-6. Epub 2007 Aug 13. Galandrini, Ricciarda| Porpora, Maria Grazia| Stoppacciaro, Antonella| Micucci, Federica| Capuano, Cristina| Tassi, Ilaria| Di Felice, Alessia| Benedetti-Panici, Pierluigi| Santoni, Angela Department of Experimental Medicine, University "La Sapienza," Rome, Italy. ricciarda.galandrini@uniroma1.it OBJECTIVE: To analyze the frequency of peritoneal natural killer (NK) cells expressing the human leukocyte antigen (HLA)-E receptor CD94/NKG2A in patients with endometriosis. DESIGN: Case-control study. SETTING: University hospital. PATIENT(S): Stage III and stage IV endometriosis, according to the revised American Society for Reproductive Medicine classification, was laparoscopically and histologically confirmed in 11 and 9 patients, respectively; 13 subjects without endometriosis were selected for the control group. INTERVENTION(S): Collection of peripheral venous blood, peritoneal fluid, endometriotic tissue, and normal endometrium in subjects undergoing laparoscopy. MAIN OUTCOME MEASURE(S): Surface expression levels of CD94/NKG2A and CD94/NKG2C were detected by three-color cytofluorometric analysis. Semiquantitative HLA-E messenger RNA expression analysis was performed in endometriotic lesions and in eutopic endometrium. NK cell-mediated cytotoxic activity toward HLA-E positive target, DT360 cell line, was also determined. RESULT(S): In women with endometriosis, the percentage of CD94/NKG2A-positive peritoneal NK cells was significantly higher than in the control group. The CD94/NKG2A ligand, HLA-E, was detected at high levels in endometriotic tissue as messenger RNA transcript. Target cells bearing HLA-E were resistant to NK cell-mediated lysis in a CD94/NKG2A-dependent manner. CONCLUSION(S): Increased expression of CD94/NKG2A in peritoneal NK cells may mediate the resistance of endometriotic tissue to NK cell-mediated lysis, thus contributing to the progression of the disease. Mesh Terms: Adult| Ascitic Fluid/*immunology/metabolism/pathology| Case-Control Studies| Disease Progression| Endometriosis/blood/*immunology/metabolism/pathology| Female| HLA Antigens/genetics/metabolism| Histocompatibility Antigens Class I/genetics/metabolis |
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